The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assays, resulting in false negatives or . objetivos: conocer las diferentes técnicas de aglutinación usadas en el laboratorio. adquirir habilidad y destrezas en las técnicas de. Prozone effect: No prozone effect was detected upon mg/dL. 4. . Efecto prozona: No se observa efecto prozona hasta valores de

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From Wikipedia, the free encyclopedia. Retrieved 3 February The eefecto effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assaysresulting in false negatives or inaccurately low results.

If the antibodies agglutinate with the antigen to form immune complexesthen the test is interpreted as positive.

Term Bank – efecto prozona – Spanish English Dictionary

Because no agglutination occurs, the test is interpreted prizona negative. Examples include high levels of syphilis antibodies in HIV patients or high levels of cryptococcal antigen leading to false negative tests in undiluted samples.


The range of relatively high antibody concentrations within which no reaction occurs is called the prozone. However, if too many antibodies are present that can bind to the antigen, then the antigenic sites are coated by antibodies, and few or no antibodies directed toward the pathogen are able to bind more than one antigenic particle. In this case, the result is a false negative. The phenomenon is caused by very high concentrations of a particular analyte or antibody and is most prevalent in one-step sandwich immunoassays.

In this case, free antigen is in competition with captured antigen for detection antibody binding. The serological test is mainly seen in the precipitation reaction.

Other common forms of interference include antibody interference, cross-reactivity and signal interference. In an agglutination test, a person’s serum which contains antibodies is added to a test tubewhich contains a particular antigen. Retrieved from ” https: Views Read Edit View history.

Has its time arrived? The antibody that fails to react is pprozona as the blocking antibody and prevents the precipitating antibody from binding to the antigens. The Clinical Biochemist Reviews.


The effect can also occur because of antigen excess, when both the capture and detection antibodies become saturated by the high analyte concentration. Thus the proper precipitation reaction does not take place.

Hook effect

This page was last edited on 14 Augustat By using this site, you agree to the Terms of Use and Privacy Policy. However, when the serum is diluted, the blocking antibody is as well and its concentration decreases enough for lrozona proper precipitation reaction to occur.

In this case, no sandwich can be formed by the capturing antibody, the antigen and the detection antibody.